1 Protein loop compaction and the origin of the effect of arginine and glutamic acid 1 mixtures on solubility , stability and transient oligomerization of proteins 2 3
نویسندگان
چکیده
Protein loop compaction and the origin of the effect of arginine and glutamic acid 1 mixtures on solubility, stability and transient oligomerization of proteins 2 3 Jascha Blobel, Ulrika Brath, Pau Bernadó, Carl Diehl, Lidia Ballester, 4 Alejandra Sornosa, Mikael Akke & Miquel Pons 5 6 1. Laboratory of Biomolecular NMR, Institute for Research in Biomedicine. Parc 7 Científic de Barcelona, Baldiri Reixac, 10, 08028 Barcelona, Spain. 8
منابع مشابه
Optimizing refolding condition for recombinant tissue plasminogen activator
Low molecular size additives such as L-arginine and the redox compounds have been used both in the culturemedium and in vitro refolding to increase recombinant proteins production. Additives increase proteinrefolding and yield of active proteins by suppressing aggregate formation or enhancing refolding process.In this work, a comparative study was performed on refolding of rec...
متن کاملA review of methods to increase the stability of recombinant pharmaceutical proteins during the production and storage process
The production of biotechnological drug proteins plays an important role against disease. The process of producing drug recombinant proteins is not a direct path, because it requires a lot of work and on the other hand, one of the important and significant aspects in the production of proteins is the discussion of their stability and solubility during the expression and purification process. Pr...
متن کاملPhysicochemical and rheological parameters changes for determining the quality of surimi and kamaboko produced by conventional, acid and alkaline solubilization process methods from common kilka (Clupeonella cultriventris caspia)
Physicochemical properties of surimi and kamaboko obtained of solubility in acid, alkaline and conventional methods were compared. The results indicated that the highest protein recovery was related to solubility in acid, alkaline and conventional methods, respectively. The highest removal of lipid and myoglobin was observed by solubility in alkali. Excretion of total pigment and sulfhydryl gro...
متن کاملComparison Studies on Complexation of Molybdenum(VI) with Aspartic Acid And Glutamic Acid in Different Dielectric Constants1
Equilibria of the reaction of molybdenum(VI) with L-aspartic acid (Asp) and L- Glutamic acid(Glu) have been studied by spectrophotometric measurements in mixed solvent systems at an ionicstrength of 0.2 mol/dm3 sodium perchlorate, employed (15, 20, 25, 30± 0.1°C) at pH ranges of 3.2to 5.5 with a high ratio of ligand to metal. The stability constants of the complexes and theresulting free energy...
متن کاملPhysicochemical and rheological parameters changes for determining the quality of surimi and kamaboko produced by conventional, acid and alkaline solubilization process methods from common kilka (Clupeonella cultriventris caspia)
Physicochemical properties of surimi and kamaboko obtained of solubility in acid, alkaline and conventional methods were compared. The results indicated that the highest protein recovery was related to solubility in acid, alkaline and conventional methods, respectively. The highest removal of lipid and myoglobin was observed by solubility in alkali. Excretion of total pigment and sulfhydryl gro...
متن کامل